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What is Tissue-Culture Grade of Water?

By Julia Oxford, Ph.D.
Professor of Biochemistry
Boise State University

   Tissue-culture grade of water is high-grade water with no impurities or contaminants. To be considered tissue-culture grade, water is tested for its ability to support cell growth and maintenance in the laboratory when used to reconstitute cell growth medium. The pH of tissue-culture grade water should be slightly acidic, between 5.7 and 7.0.

   Inorganic minerals, essential for cell growth, must be present in minute quantities. Organic matter is absent, including natural and man-made organic molecules such as proteins, pesticides, herbicides, tannins and detergents. Microorganisms such as bacteria, viruses and algae are absent, as are organic breakdown products. The water is of low toxicity and endotoxin free.

   Bio-Logic Aqua Absolute Premium Standard™ tissue-culture grade water, the ingredient in all Bio-Logic Aqua Technologies products, meets or surpasses the above criteria and has a natural pH of 6.4.
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